New Tutorial: Object tracking using CellProfiler

Author(s)

Beatriz Serrano-Solano

Yi Sun

Jean-Karim Hériché

Posted on: 18 March 2021

Most biological processes are dynamic and observing them over time can provide valuable insights. Combining fluorescent markers with time-lapse imaging is a common approach to collect data on dynamic cellular processes such as cell division. However, automated time-lapse imaging can produce large amounts of data that can be challenging to process. One of these challenges is the tracking of individual objects as it is often impossible to manually follow a large number of objects over many time points.

To demonstrate how automatic tracking can be applied in such situations, this tutorial will track dividing nuclei in a short time-lapse recording of one mitosis of a syncytial blastoderm stage Drosophila embryo expressing a GFP-histone gene that labels chromatin.

View Material

Video of green coloured cells dividing on a dark background.

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